张传健;刘娅梅;侯继波;王继春;

• 1:江苏省农业科学院动物免疫工程研究所
• 2:江苏省农业科学院国家兽用生物制品工程技术研究中心
• 3:江苏省动物重要疫病与人兽共患病防控协同创新中心

摘要(Abstract)：

伪狂犬病病毒(PRV)属于疱疹病毒,含有多个复制非必需区,可以通过基因缺失研制弱毒疫苗,也可以通过异源基因的插入或替换构建活载体疫苗。目前构建重组PRV的技术方法包括传统同源重组技术、细菌人工染色体技术(BAC)、CRISPR/Cas9介导的基因编辑技术。2011以来,一种毒力更强的变异株在我国暴发流行,应用变异株构建的基因缺失活疫苗(gE/gI、gE /TK或者TK/gE/gI)能对变异株提供完全的保护,为PRV在我国的净化和PRV变异株活载体疫苗的构建奠定了基础。研究发现以PRV基因缺失株为载体,与其他病原的抗原编码基因共同构建重组病毒,能快速地诱导细胞免疫和体液免疫,达到一针多防的效果,应用前景广阔。适合的插入位点选择、外源基因高效表达和克服PRV母源抗体干扰等是提高活载体效果的关键。

关键词(KeyWords)： 伪狂犬病病毒;重组病毒;载体;外源基因

Abstract:

Keywords:

基金项目(Foundation): 江苏省博士后科研基金资助项目(2018K205C);; 江苏省农业科技自主创新基金项目[CX(18)3020];; 江苏省自然科学基金(BK20181234)

作者(Author): 张传健;刘娅梅;侯继波;王继春;

Email:

DOI:

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